سوخت و ساز و فعالیت ورزشی

شماره جاری

بر اساس شماره‌های نشریه

بر اساس نویسندگان

بر اساس موضوعات

نمایه نویسندگان

نمایه کلیدواژه ها

درباره نشریه

اهداف و چشم انداز

سیاست دسترسی آزاد

اعضای هیات تحریریه

اصول اخلاقی انتشار مقاله

سیاست شورای سردبیری و مجله

بانک ها و نمایه نامه ها

فرایند پذیرش مقالات

پیوندهای مفید

پرسش‌های متداول

اخبار و اعلانات

لینک صفحات مجله در شبکه های اجتماعی

راهنمای نویسندگان

فرم ها

راهنمای عضویت و ثبت داوری در Publons

لیست داوران سال 1401

لیست داوران سال 1400

تغییرات سطح پروتئین شبه آنژیوپویتین 4 و مقاومت به انسولین در موش های مبتلا به کبد چرب غیر الکلی: تأثیر تمرین تناوبی شدید و عصاره خرفه

نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران

2 گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران.

چکیده

هدف از تحقیق حاضر بررسی تأثیر 8 هفته تمرین تناوبی شدید (HIIT) همراه با مصرف مکمل گیاه خرفه بر سطح پروتئین شبه آنژیوپویتین 4 (ANGPTL4) کبدی و همچنین مقاومت به انسولین در موشهای مبتلا به NAFLD بود.

بدین منظور 25 سر موش صحرایی نر بالغ نژاد ویستار با سن شش هفته و با دامنه ی وزنی 160 تا 185 گرم، به طور تصادفی انتخاب و در 5 گروه کنترل سالم، کنترل کبد چرب، مصرف مکمل خرفه، تمرین HIIT و تمرین همراه با مصرف مکمل خرفه قرار داده شدند.جهت القای NAFLD ، موشها به مدت 12هفته تحت رژیم غذایی پُرچرب قرار گرفتند. مکمل خرفه با توجه به وزن موشها با دوز mg/kg 400 به گروههای مربوطه خورانده شد. پروتکل تمرینی HIIT به مدت 8 هفته، 5 جلسه در هفته با شدت ۹0درصد سرعت بیشینه انجام شد.

مقاومت به انسولین درگروه کنترل کبد چرب بطور معناداری نسبت به سالم کنترل بالاتر بود (002/0=p). سطح این شاخص در گروه تمرین HIIT (01/0=p)، مکمل خرفه (037/0=p) و تمرین همراه با مکمل (012/0=p) به طور معناداری پایینتر از گروه کنترل کبدچرب بود. همچنین سطح ANGPTL4 کبدی در گروه کنترل کبدچرب بطور معناداری نسبت به سالم کنترل بالاتر بود (01/0=p). سطح این شاخص در گروه تمرین HIIT (036/0=p)، مصرف مکمل خرفه (01/0=p) و تمرین همراه بامکمل (007/0=p) بطور معناداری پایینتر از گروه کنترل کبدچرب بود.

احتمالا تمرین HIIT به تنهایی و همراه بامصرف مکمل خرفه منجر به بهبود مقاومت به انسولین و ANGPTL4 می شود بنابراین می توانند در کنترل بیماری کبدچرب نقش کلیدی داشته باشند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Changes in Angiopoietin-like 4 Protein Level and Insulin Resistance in Rats with Non-Alcoholic Fatty Liver Disease: Effect of High Intensity Interval Training and Portulaca Oleracea extract

نویسندگان [English]

  • Somayeh Kazemi-Esfahani 1
  • Ali Yaghoubi 2

1 Department of Physical Education and Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran.

2 Department of Physical Education and Sport Science, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran.

چکیده [English]

Introduction: The aim of this study was to investigate the effect of eight weeks of high intensity interval training (HIIT) and Portulaca Oleracea extract on Angiopoietin-like 4 Protein (ANGPTL4) level and insulin resistance in rats with NAFLD.

Methods: Twenty five male Wistar rats were randomly divided into five groups: healthy control, fatty liver control, HIIT, Portulaca Oleracea extract, and HIIT+Portulaca Oleracea extract. To induce NAFLD, the rats were fed a high-fat diet for 12 weeks. Portulaca Oleracea supplement at 400 mg/kg was given to the experimental groups. HIIT was performed for 8 weeks, 5 sessions per week at 90% maximum speed. Hepatic ANGPTL4 level and insulin resistance were measured after 8 weeks of HIIT and consumption of purslane extract. One way anova and tukey post hoc test was used for data analysis.

Results: Insulin resistance was significantly higher in fatty liver control group than healthy controls (P=0.002). But it was significantly lower in HIIT group (P=0.01), Portulaca Oleracea extract (P=0.037), and HIIT+Portulaca Oleracea extract group (P=0.012) than that in fatty liver control group. ANGPTL4 levels in the healthy control group was significantly higher than fatty liver control group (p=0.01). levels of this index in HIIT group (p=0.036), Portulaca Oleracea extract (p=0.01) and HIIT+ Portulaca Oleracea extract (p=0.007) was significantly lower than the fatty liver control group.

Conclusion: It seems that HIIT and Portulaca Oleracea extract can play an important role in controlling the progression of this disease by reducing the level of ANGPTL4 and insulin resistance in patients with NAFLD.

کلیدواژه‌ها [English]

  • High intensity interval training
  • Portulaca Oleracea extract
  • ANGPTL4
  • insulin resistance
  • non-alcoholic fatty liver disease
مراجع
  1. Khorasani S, Azizi H, Yousefi M, Salari R, Bahrami-Taghanaki H, Behravanrad P. An evidence-based review on integrative medicine in weight control. Complementary Medicine Journal. 2017;7(1):1828-50.
  2. Safarpor M, Kohan L, Porkhajeh A. Comparative study of anthropometric parameters in non-alcoholic fatty liver disease patients and healthy subjects. Journal of Sabzevar University of Medical Sciences. 2015;22(3):225-31.
  3. Behzadimoghadam M, Galedari M, Motalebi L. The effect of eight weeks resistance training and low-calorie diet on plasma levels of liver enzymes and liver fat in Non-Alcoholic Fatty Liver Disease (NAFLD). Iranian Journal of Nutrition Sciences & Food Technology. 2018;12(4):25-32.
  4. Skrypnik D, Ratajczak M, Karolkiewicz J, Mądry E, Pupek-Musialik D, Hansdorfer-Korzon R, et al. Effects of endurance and endurance–strength exercise on biochemical parameters of liver function in women with abdominal obesity. Biomedicine & Pharmacotherapy. 2016; 80:1-7.
  5. Keating SE, Hackett DA, Parker HM, O’Connor HT, Gerofi JA, Sainsbury A, et al. Effect of aerobic exercise training dose on liver fat and visceral adiposity. Journal of hepatology. 2015;63(1):174-82.
  6. Schultz A, Mendonca LS, Aguila MB, Mandarim-de-Lacerda CA. Swimming training beneficial effects in a mice model of nonalcoholic fatty liver disease. Experimental and toxicologic pathology. 2012;64(4):273-82.
  7. Slentz CA, Bateman LA, Willis LH, Shields AT, Tanner CJ, Piner LW, et al. Effects of aerobic vs. resistance training on visceral and liver fat stores, liver enzymes, and insulin resistance by HOMA in overweight adults from STRRIDE AT/RT. American Journal of Physiology-Endocrinology and Metabolism. 2011;301(5): E1033-E9.
  8. Thoma C, Day CP, Trenell MI. Lifestyle interventions for the treatment of non-alcoholic fatty liver disease in adults: a systematic review. Journal of hepatology. 2012;56(1):255-66.
  9. Hickman IJ, Macdonald GA. Impact of diabetes on the severity of liver disease. The American journal of medicine. 2007;120(10):829-34.
  10. Aroner SA, Mukamal KJ, St-Jules DE, Budoff MJ, Katz R, Criqui MH, et al. Fetuin-A and risk of diabetes independent of liver fat content: the multi-ethnic study of atherosclerosis. American journal of epidemiology. 2017;185(1):54-64.
  11. Semmler G, Datz C, Reiberger T, Trauner M. Diet and exercise in NAFLD/NASH: Beyond the obvious. Liver International. 2021;41(10):2249-68.
  12. Hallsworth K, Trenell M. Physical Activity in NAFLD: What and How Much? Non-Alcoholic Fatty Liver Disease: Springer; 2020. p. 289-307.
  13. Guo L, Li S-Y, Ji F-Y, Zhao Y-F, Zhong Y, Lv X-J, et al. Role of Angptl4 in vascular permeability and inflammation. Inflammation Research. 2014;63(1):13-22.
  14. Merkel M, Eckel RH, Goldberg IJ. Lipoprotein lipase. Journal of lipid research. 2002;43(12):1997-2006.
  15. Le Jan S, Amy C, Cazes A, Monnot C, Lamandé N, Favier J, et al. Angiopoietin-like 4 is a proangiogenic factor produced during ischemia and in conventional renal cell carcinoma. The American journal of pathology. 2003;162(5):1521-8.
  16. Wang Y, Liu LM, Wei L, Ye WW, Meng XY, Chen F, et al. Angiopoietin-like protein 4 improves glucose tolerance and insulin resistance but induces liver steatosis in high-fat-diet mice. Molecular Medicine Reports. 2016;14(4):3293-300.
  17. Ma Y, Wang Y, Wang X, Yang L, Lang J, Zhao D. 1884-P: Serum ANGPTL2, ANGPTL3, and ANGPTL4 as potential biomarkers for diagnosis of nonalcoholic fatty liver disease. Diabetes. 2019;68(Supplement_1).
  18. Domínguez-Pérez GA, Vera-Gómez E, Hernández-Patricio A, Gutiérrez-Buendía JA, De la Vega-Moreno K, Zamora-Alemán CR, et al. Relation of ANGPTL4 with the Concentration of LPL and Hepatic Fibrosis in Patients with NAFLD. Metabolism-Clinical and Experimental. 2020;104.
  19. Trapp EG, Chisholm DJ, Freund J, Boutcher SH. The effects of high-intensity intermittent exercise training on fat loss and fasting insulin levels of young women. International journal of obesity. 2008;32(4):684-91.
  20. Gibala MJ. High-intensity interval training: a time-efficient strategy for health promotion? Current sports medicine reports. 2007;6(4):211-3.
  21. Karami M, Ghafari M, Banitalebi E. Comparison of the Effect of Two Personalized Low Volume-High Intensity and Combined (Strength-Aerobic) Exercises on Angiopoietin-like Protein 4 (ANGPTL4) Serum Levels in Women with Type 2 Diabetes: A Short Report. JRUMS 2020; 19 (1) :97-106.
  22. Changizi-Ashtiyani S, Zarei A, Taheri S, Rasekh F, Ramazani M. The effects of Portulaca oleracea alcoholic extract on induced hypercholesteroleomia in rats. Zahedan J Res Med Sci. 2013;15(6):34-9.
  23. Miladi-Gorji H, Vafaei AA, Taherian AA, Vaezi T. The effects of aqueous extracts of Purtulaca oleracea on withdrawal syndrome in mice. Journal of Medicinal Plants. 2009:51-7.
  24. Sahebkar KM, Azizi H, Yousefi M, Salari R, Bahrami TH, Behravanrad P. An evidence-based review on integrative medicine in weight control. 2017.
  25. Gatreh-Samani K, Farrokhi E, Khalili B, Rafieian M, Moradi M. Purslane (Portulaca oleracea) effects on serum paraoxanase-1 activity. Journal of Shahrekord University of medical sciences. 2011;13(1):9-14.
  26. Barjasteyazdy A, Zarei M. The effect of high intensity interval training (HIIT) with portulaca Oleracea supplementation on serum levels of liver enzyme in rats with non-alcoholic fatty live disease. Journal of Sport and Biomotor Sciences. 2021;26(26):56-65.
  27. Hafstad AD, Lund J, Hadler-Olsen E, Höper AC, Larsen TS, Aasum E. High-and moderate-intensity training normalizes ventricular function and mechanoenergetics in mice with diet-induced obesity. Diabetes. 2013;62(7):2287-94.
  28. Hafstad AD, Boardman NT, Lund J, Hagve M, Khalid AM, Wisløff U, et al. High intensity interval training alters substrate utilization and reduces oxygen consumption in the heart. Journal of Applied Physiology. 2011;111(5):1235-41.
  29. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Preventive Cardiology. 2007;14(6):753-60.
  30. Darvish Damavandi R, Shidfar F, Najafi M, Janani L, Masoodi M, Akbari‐Fakhrabadi M, et al. Effect of Portulaca Oleracea (purslane) extract on liver enzymes, lipid profile, and glycemic status in nonalcoholic fatty liver disease: A randomized, double‐blind clinical trial. Phytotherapy Research. 2021.
  31. Zarei A, Changizi Ashtiyani S, Taheri S. The effects of hydroalcoholic extract of Portulaca Oleracea on the serum concentreation of Hepatic enzymes in Rats. ISMJ. 2014;17(5):889-99.
  32. Kersten S, Lichtenstein L, Steenbergen E, Mudde K, Hendriks HF, Hesselink MK, et al. Caloric restriction and exercise increase plasma ANGPTL4 levels in humans via elevated free fatty acids. Arteriosclerosis, thrombosis, and vascular biology. 2009;29(6):969-74.
  33. Smart-Halajko MC, Robciuc MR, Cooper JA, Jauhiainen M, Kumari M, Kivimaki M, et al. The relationship between plasma angiopoietin-like protein 4 levels, angiopoietin-like protein 4 genotype, and coronary heart disease risk. Arteriosclerosis, thrombosis, and vascular biology. 2010;30(11):2277-82.
  34. Lu B, Moser A, Shigenaga JK, Grunfeld C, Feingold KR. The acute phase response stimulates the expression of angiopoietin like protein 4. Biochemical and biophysical research communications. 2010;391(4):1737-41.
  35. Ryan BJ, Schleh MW, Ahn C, Ludzki AC, Gillen JB, Varshney P, et al. Moderate-intensity exercise and high-intensity interval training affect insulin sensitivity similarly in obese adults. The Journal of Clinical Endocrinology & Metabolism. 2020;105(8): e2941-e59.
  36. Henstridge DC, Forbes JM, Penfold SA, Formosa MF, Dougherty S, Gasser A, et al. The relationship between heat shock protein 72 expression in skeletal muscle and insulin sensitivity is dependent on adiposity. Metabolism. 2010;59(11):1556-61.
  37. Kavanagh K, Davis AT, Jenkins KA, Flynn DM. Effects of heated hydrotherapy on muscle HSP70 and glucose metabolism in old and young vervet monkeys. Cell Stress Chaperones. 2016;21(4):717-25.
  38. Schenk S, Horowitz JF. Acute exercise increases triglyceride synthesis in skeletal muscle and prevents fatty acid–induced insulin resistance. The Journal of clinical investigation. 2007;117(6):1690-8.
  39. Turcotte LP, Fisher JS. Skeletal muscle insulin resistance: roles of fatty acid metabolism and exercise. Physical therapy. 2008;88(11):1279-96.
  40. Darvish Damavandi R, Shidfar F, Najafi M, Janani L, Masoodi M, Akbari‐Fakhrabadi M, et al. Effect of Portulaca Oleracea (purslane) extract on liver enzymes, lipid profile, and glycemic status in nonalcoholic fatty liver disease: A randomized, double‐blind clinical trial. Phytotherapy Research. 2021;35(6):3145-56.
  41. Hingst JR, Onslev JD, Holm S, Kjøbsted R, Frøsig C, Kido K, et al. Insulin Sensitization Following a Single Exercise Bout Is Uncoupled to Glycogen in Human Skeletal Muscle: A Meta-analysis of 13 Single-Center Human Studies. Diabetes. 2022;71(11):2237-50.
  42. Newsom SA, Schenk S, Thomas KM, Harber MP, Knuth ND, Goldenberg N, et al. Energy deficit after exercise augments lipid mobilization but does not contribute to the exercise-induced increase in insulin sensitivity. Journal of applied physiology. 2010; 108(3):554-60.
  43. Gauthier M, Couturier K, Charbonneau A, Lavoie J. Effects of introducing physical training in the course of a 16-week high-fat diet regimen on hepatic steatosis, adipose tissue fat accumulation, and plasma lipid profile. International journal of obesity. 2004;28(8):1064-71.
  44. Cook JJ, Wei M, Segovia B, Cosio-Lima L, Simpson J, Taylor S, et al. Endurance exercise-mediated metabolic reshuffle attenuates high-caloric diet-induced non-alcoholic fatty liver disease. Annals of Hepatology. 2022;27(4):100709.
  45. Takahashi A, Abe K, Usami K, Imaizumi H, Hayashi M, Okai K, et al. Simple resistance exercise helps patients with non-alcoholic fatty liver disease. International journal of sports medicine. 2015;94(10):848-52.
  46. Uddin M, Juraimi AS, Hossain MS, Nahar M, Un A, Ali M, et al. Purslane weed (Portulaca oleracea): a prospective plant source of nutrition, omega-3 fatty acid, and antioxidant attributes. The Scientific World Journal. 2014;2014.
  47. Jeyapal S, Kona SR, Mullapudi SV, Putcha UK, Gurumurthy P, Ibrahim A. Substitution of linoleic acid with α-linolenic acid or long chain n-3 polyunsaturated fatty acid prevents Western diet induced nonalcoholic steatohepatitis. Scientific reports. 2018;8(1):1-14.
  48. Brands M, Sauerwein HP, Ackermans MT, Kersten S, Serlie MJ. Omega-3 long-chain fatty acids strongly induce angiopoietin-like 4 in humans. J Lipid Res. 2013;54(3):615-21.
  49. Ebrahimian Z, Razavi BM, Mousavi Shaegh SA, Hosseinzadeh H. Effects of Portulaca oleracea L.(purslane) on the metabolic syndrome: A review. Iranian Journal of Basic Medical Sciences. 2022;25(11): 1275-1285.
  50. Bai Y, Zang X, Ma J, Xu G. Anti-diabetic effect of Portulaca oleracea L. Polysaccharideandits mechanism in diabetic rats. International journal of molecular sciences. 2016;17(8):1201.
  51. Gavaher MA, Babazadeh D, Sadeghi A, Hejazi V, Sasani F, Moshavery A, et al. Comparative Assessment of Portulaca oleracea, Omega-3 Fatty Acids, and Combination of Selenium Plus Vitamin E on Histopathology of Pancreas in Diabetic Rats. Journal of Veterinary Physiology and Pathology. 2022;1(1):17-23.
  52. Rakhshandeh H, Rajabi Khasevan H, Saviano A, Mahdinezhad MR, Baradaran Rahimi V, Ehtiati S, et al. Protective Effect of Portulaca oleracea on Streptozotocin-Induced Type I Diabetes-Associated Reproductive System Dysfunction and Inflammation. Molecules. 2022;27(18):6075.
  53. Ramadan BK, Schaalan MF, Tolba AM. Hypoglycemic and pancreatic protective effects of Portulaca oleracea extract in alloxan induced diabetic rats. BMC complementary and alternative medicine. 2017;17(1):1-10.
  54. Lan S, Fu-er L. Effects of Portulaca oleracea on insulin resistance in rats with type 2 diabetes mellitus. Chinese Journal of Integrative Medicine. 2003;9(4):289-92.
  55. Hadi A, Pourmasoumi M, Najafgholizadeh A, Kafeshani M, Sahebkar A. Effect of purslane on blood lipids and glucose: A systematic review and meta‐analysis of randomized controlled trials. Phytotherapy research. 2019;33(1):3-12.
  56. Simopoulos AP, Norman HA, Gillaspy JE, Duke JA. Common purslane: a source of omega-3 fatty acids and antioxidants. Journal of the American College of Nutrition. 1992;11(4):374-82.
  57. Farzanegi P. Impact of the Synchronization of portulaca oleracea and Aerobic Training on Levels of MMP2 and MMP9 and TIMP1 in Diabetic Women Type II. Res-Mol-Med. 2014;2(2):34-9.
  58. El-Sayed M-IK. Effects of Portulaca oleracea L. seeds in treatment of type-2 diabetes mellitus patients as adjunctive and alternative therapy. Journal of Ethnopharmacology. 2011;137(1):643-51.
سوخت و ساز و فعالیت ورزشی
دوره 12، شماره 1 - شماره پیاپی 1
فروردین 1401
صفحه 141-158
فایل ها
سابقه مقاله
  • تاریخ دریافت: 23 اسفند 1401
  • تاریخ بازنگری: 27 اسفند 1401
  • تاریخ پذیرش: 27 اسفند 1401
هم رسانی
ارجاع به این مقاله
آمار