نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I


1 گروه فیزیولوژی ورزشی و حرکات اصلاحی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران

2 گروه کلینیکال پاتولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران


هدف از پژوهش حاضر بررسی تاثیر هشت تمرین هوازی با شدت متوسط با و بدون مصرف مکمل نانوذره و ذره‌نمکی اکسید روی بر شاخصهای اکسیداتیو بافت کبد موش های آلوده شده با BPA بود.

مواد و روش‌ها: 60 سر موش در 12 گروه 5 تایی: 1) کنترل، 2) BPA، 3) تمرین هوازی، 4) تمرین هوازی+BPA، 5) نانوذره، 6) نانوذره+BPA، 7) ذره نمکی اکسید روی، 8) ذره نمکی اکسید روی+BPA، 9) تمرین+ نانوذره، 10) تمرین+ نانوذره+BPA، 11) تمرین+ذره نمکی اکسید روی و 12) تمرین+ذره نمکی اکسید روی+BPA قرار گرفتند. تمرین هوازی با شدت 75ـ50 درصد VO2max و به مدت 64ـ25 دقیقه اجرا شد. مکمل‌ روی تا اتمام دوره تزریق شد.

نتایج: فعالیت آنزیم‌های GPX، SOD و کاتالاز در گروه تمرین ورزشی و گروه‌‌های BPA افزایش معنی‌داری نشان داد، در بررسی مقادیر TAC مشخص شد تمرین ورزشی به تنهایی سبب کاهش معنی‌دار (001/0=P) و گروه‌های دریافت کننده مکمل‌های Zno و NanoZno سبب افزایش معنی‌دار این شاخص نسبت به گروه کنترل سالم شد (001/0p=). این در حالی بود که در بررسی MDA و AOPP مشخص شد گروه‌های BPA افزایش شدیدی در این متغیرها ایجاد کردند که مکمل سبب کاهش معنی‌دار این متغیرها شد و بیشترین کاهش مربوط به BPA+Nano و BPA+Zno بود (001/0p= ).

نتیجه‌گیری: دریافت BPA ظرفیت آنتی‌اکسیدانتی را تخریب می‌نماید. با وجود این، ترکیب تمرین ورزشی با شدت متوسط و مکمل‌های اکسید روی (به ویژه مکمل به تنهایی) ممکن است کینتیک آلوده‌شدن با BPA را با مهار سیستم اکسیدانی تغییر دهد و متعاقب آن بهبود وضعیت هپاتوسیت‌ها را بدنبال داشته باشد.



عنوان مقاله [English]

Response of Liver Tissue Oxidative Indices to Bisphenol-A intoxicated Rats to Eight Weeks of Aerobic Training with and without Zinc Supplementation (Zinc Nanoparticles and Nanoparticles)

نویسندگان [English]

  • Mohammad Salmasi 1
  • Asghar Tofighi 1
  • Siamak Asri 2
  • Javad Tolouei Azar 1

1 Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, Iran

2 Department of Internal Diseases and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

چکیده [English]

Aims: The purpose of this study was to investigate the effect of moderate-intensity aerobic exercise with and without supplementation of nanoparticles and zinc oxide salts on hepatocyte oxidative markers in BPA-intoxicated rats.

Methods: 60 male rats were divided into 12 groups: 1) Control; 2) BPA; 3) Exercise; 4) Exercise+BPA; 5) Nanoparticle supplementation (Nano); 6) Nanoparticle+BPA (Nano+BPA); 7) Zinc oxide salt (Zno); 8) Zinc oxide salt+BPA (Zno+BPA); 9) Exercise+Nano; 10) Exercise+Nano+BPA; 11) Exercise+Zno; and 12) Exercise+Zno+BPA. The exercise program was performed for eight weeks with 50-75% VO2max for 25 to 64 minutes. Nanoparticles supplemented give with 5 mg/kg 5 days a week for eight weeks.

Results: The activity of the antioxidant enzymes GPX, SOD and Catalase increase significantly in the BPA group in addition to the increase in the exercise group, but TAC status decreased significantly in the exercise group alone (p = 0.001) and Zno and NanoZno groups significantly increased this index compared to the healthy control group (p = 0.001). Interestingly, in MDA and AOPP, the BPA groups showed a significant increase in these variables, supplementation caused a significant decrease in these variables, and the highest reduction was in BPA+Nano and BPA+Zno (p = 0.001).

Conclusion: BPA causes reactive changes in antioxidant capacity, it destroys total antioxidant capacity. However, oxidative index studies revealed that a combination of moderate-intensity exercise and zinc oxide supplements (especially supplementation alone) may alter the kinetics of BPA contamination by inhibiting the oxidative system and subsequently improving hepatocyte status.

کلیدواژه‌ها [English]

  • Exercise training"
  • Zinc"
  • Bisphenol-A"
  • Oxidative markers"
  • "
  • Hepatocyte"
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