نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 عضو هیات علمی دانشگاه هنر اسلامی تبریز
2 گروه فیزیولوژی ورزشی، دانشکده‌ی تربیت بدنی علوم ورزشی، دانشگاه تبریز، تبریز، ایران
چکیده
مقدمه: هدف از پژوهش حاضر بررسی تأثیر شبیه سازی ماندن در ارتفاع متوسط به همراه مصرف آویشن بر میزان کاردیومیوپاتی موش‌های صحرایی دیابتی نوع دو و تأثیر همزمان آن‌ها بر آنزیم‌های کبدی بود. روش‌ها: بدین منظور تعداد 40 سر موش صحرایی به پنج گروه هشت تایی تقسیم شدند. جهت القای دیابت نوع دو استرپتوزوتوسین با دوز mg/kg37 تزریق شد. گروه ارتفاع هشت هفته در شرایط شب مانی در ارتفاع شبیه سازی شده در اتاقک هیپوکسی (14% معادل فشار اکسیژن 106میلیمتر جیوه و مشابه ارتفاع 3400متر) بطور متناوب قرار گرفتند و گروه مکمل یاری آویشن بصورت mg/kg 400 عصاره هیدروالکلی در 30ml آب مصرفی بصورت روزانه دریافت کردند. کاردیومیوپاتی از طریق دو شاخص آپوپتوز (وسترن بلات)و فیبروز (رنگ آمیزی) میوکارد اندازه گیری شد و آنزیم‌های کبدی با استفاده از روش‌های کالری متری شرکت زیست شیمی اندازه گیری گردید. یافته‌ها: نتایج نشان داد 8 هفته شب مانی در ارتفاع، مکمل یاری آویشن و ترکیب این دو موجب کاهش میزان گلوکز ناشتا در دیابتی‌ها می‌شود (05/0>P). همچنین آپوپتوز و فیبروز با قرارگرفتن در ارتفاع و مصرف همزمان آویشن در میوکارد کاهش معنی‌داری در دیابت نوع دو نشان داد (0.025p<). همچنین ارتفاع متوسط و ﻣﺼﺮف ﻋﺼﺎره آویشن ﺗﺄﺛﯿﺮ ﻣﻌﻨﯽداری ﺑﺮ سطوح سرمی ترانس‌آمینازهای ALT و AST نداشت.
نتیجه‌گیری: ارتفاع شبیه‌سازی شده متوسط و آویشن در این تحقیق موجب کاهش قند خون ناشتا شد و از طرفی با کاهش آپوپتوز و فیبروز موجب پیشگیری از کاردیومیو پاتی موش‌های صحرایی دیابتی نوع دو شد اما این دو متغیر موجب کاهش معنی‌دار آنزیم‌های ALT و AST نشدند.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The effect of simulating staying at moderate altitude with thyme consumption on cardiomyopathy and liver enzymes in Rats with type 2 diabetes
نویسندگان [English]
- mahdi faramoushi 1
- Ramin Amirsasan 2
- Vahid sari sarraf 2
1 هیات علمی
2 Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz
چکیده [English]
the aim of study was to investigate the effect of simulating staying at medium altitude along with consumption of thyme on the cardiomyopathy rate of type 2 diabetic rats and their simultaneous effect on liver enzymes.Methods: For this purpose, 40 Wistar rats were divided into five groups of eight. In order to induce type 2 diabetes, streptozotocin was injected intraperitoneally.Altitude group was alternately placed for eight weeks in night sleep conditions at a simulated altitude in a hypoxia chamber, and thyme group was supplemented with thyme in the form of 400 mg/kg of hydroalcoholic extract in 30 ml of drinking water. Cardiomyopathy was measured through two indices of apoptosis (western blot) and fibrosis (staining), and diabetic indices, liver enzymes using methods Calorimetry was measured by biochemistry company.
Results: The results showed that 8 weeks of altitude and thyme reduce the fasting glucose level in diabetics (P<0.05). Also, the results of the multivariate analysis of variance test showed Apoptosis and fibrosis showed a significant decrease in myocardial with exposure to altitude and simultaneous consumption of thyme (p<0.025). Also, moderate altitude and consumption of thyme extract had no significant effect on the serum levels of ALT and AST transaminases.
Conclusion: Simulated moderate altitude and consumption of thyme in this research decreased fasting blood sugar and on the other hand, by reducing apoptosis and fibrosis, it prevented cardiomyopathy in the heart of type 2 diabetic rats, but these two variables did not cause a significant decrease in ALT and AST enzymes.
کلیدواژه‌ها [English]
- moderate altitude
- Thyme
- Cardiomyopathy
- Liver enzymes
- Type II diabetes
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