نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی بجنورد، بجنورد، ایران
2 گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی بجنورد، بجنورد، ایران.
چکیده
مقدمه: یکی از اختلالات شایع در دوره سالمندی آتروفی عضلانی وابسته به سن یا همان سارکوپنیا می‌باشد که منجر به کاهش توده عضلانی در سالمندی می‌شود. هدف از این پژوهش، بررسی اثر تمرینات مقاومتی و استقامتی بر بیان ژن‌های MURF1 و MTOR در رت‌های نر سالمند بود.. روش: تعداد ۳۰ سر موش صحرایی نر سالمند، با سن ۱۸ ماه به طور تصادفی به ۳ گروه کنترل (۱۰سر)، تمرین استقامتی (۱۰ سر) و تمرین مقاومتی (۱۰ سر) تقسیم شدند. در گروه استقامتی تمرینات به مدت ۸ هفته و ۵ جلسه در هفته بر روی نوارگردان اجرا شد و گروه مقاومتی تمرینات را به مدت ۸ هفته و ۵ جلسه در هفته بر روی نردبان انجام دادند. ۴۸ ساعت پس از آخرین جلسه تمرین و پس از ناشتایی شبانه، تمامی موش های صحرایی نر سالمند کشته شدند و از عضله دوقلو بافت‌برداری صورت گرفت. سپس میزان بیان ژن های MURF1 و mTOR با استفاده از ژن مرجع به روش Real Time PCR اندازه‌گیری شد. تجزیه و تحلیل داده‌ها توسط آزمون واریانس یک‌طرفه و آزمون تعقیبی توکی و با استفاده از نرم افزار آمارSPSS صورت گرفت. آزمون آنالیز واریانس نشان داد که میزان بیان ژن‌های mTOR و MURF1 در گروه‌های مختلف تفاوت معنی‌داری دارد (05/0≥P). یافته‌ها: نتایج آزمون تعقیبی توکی نشان داد بین گروه کنترل و مقاومتی تفاوت معنی‌داری وجود دارد و تمرین مقاومتی تاثیر بیشتری نسبت به تمرین استقامتی دارد.. نتیجه‌گیری: مطالعه حاضر نشان داد تمرینات مقاومتی نسبت به تمرینات استقامتی تاثیر بیشتری در فعال‌سازی مسیرهای اتوفاژی در عضله اسکلتی دارند. بنابراین، ممکن است تمرین مقاومتی بتواند با بهبود عوامل مرتبط با آتروفی عضلانی، پیشرفت سارکوپنیا در دوره سالمندی را مهار نماید.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The effect of resistance and endurance training on the expression of MURF1 and MTOR genes in aged male rats
نویسندگان [English]
- sadegh cheragh birjandi 1
- somaye rostamian dolat shanlo 2
- Ali yaghobi 1
1 Assistant Professor, Department of Physical Education and Sport Sciences, Bojnourd Islamic Azad University, Bojnourd, Iran.
2 Department of Physical Education and Sport Sciences, Bojnourd Islamic Azad University, Bojnourd, Iran
چکیده [English]
Introduction: One of the common disorders in old age is age-related muscle atrophy or sarcopenia, which leads to a decrease in muscle mass in old age. The aim of this study was to investigate the effect of resistance and endurance training on the expression of MURF1 and MTOR genes in aged male rats. Methods: 30 elderly male rats, aged 18 months, were randomly divided into 3 control groups (10), endurance training (10) and resistance training (10). In the endurance group, the exercises were performed on the treadmill for 8 weeks and 5 sessions per week, and the resistance group performed the exercises on the ladder for 8 weeks and 5 sessions per week. 48 hours after the last training session and after overnight fasting, all the aged male rats were killed and tissue was removed from the biceps muscle. Then, the expression level of MURF1 and mTOR genes was measured using the reference gene by Real Time PCR method. Data analysis was done by one-way variance test and Tukey's post hoc test using SPSS software. Analysis of variance test showed that the expression levels of mTOR and MURF1 genes are significantly different in different groups (P≤0.05). Results: The results of Tukey's post hoc test showed that there is a significant difference between the control and resistance groups, and resistance training has a greater effect than endurance training. The present study showed that resistance training has a greater effect on the activation of autophagy pathways in skeletal muscle than endurance training. Conclusions: Therefore, it is possible that resistance training can inhibit the development of sarcopenia in old age by improving factors related to muscle atrophy.
کلیدواژه‌ها [English]
- resistance training
- endurance training
- sarcopenia
- aging
- MURF1 and MTOR
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