نوع مقاله : مقاله پژوهشی Released under (CC BY-NC) license I Open Access I
نویسندگان
1 استادیار فیزیولوژی ورزشی، گروه علوم ورزشی، دانشگاه صنعتی شاهرود، شاهرود، سمنان.
2 استاد فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی دانشگاه شهید بهشتی، تهران- ایران.
3 دانشیار فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی دانشگاه خوارزمی تهران
چکیده
مقدمه: با عنایت به اثرات مختلف مکمل ریبوز، هدف از این پژوهش بررسی تعیین اثر مصرف حاد ریبوز بر ظرفیت ضداکسایشی تام، پراکسیداسیون لیپیدی و گزانتین اکسیداز پس از فعالیت‌های شدید تکراری بود. مواد و روش‌ها: بدین منظور ١٠ نفر از کشتی‌گیران با میانگین سن (3±22سال) و شاخص توده‌ی بدن (3±23کیلوگرم بر مترمربع) با سابقه‌ی حداقل پنج سال شرکت در تمرینات منظم کشتی، به‌طور داوطلبانه در این تحقیق شرکت کردند. تحقیق به صورت دوسوکور و متقاطع انجام شد. پروتکل شامل ٤ مرحله با فاصله ١٥دقیقه استراحت فعال بود؛ هر مرحله نیز شامل ٣ آزمون وینگیت پا و ٣ آزمون وینگیت دست به صورت متناوب و با استراحت فعال یک دقیقه‌ای بین دو آزمون بود. آزمودنی‌ها در هر دو جلسه (مصرف ریبوز یا دارونما) یک ساعت قبل از انجام کلیه‌ی مراحل آزمون اولین نوبت مصرف مکمل ریبوز (١/.گرم به ازای هر کیلوگرم از وزن بدن) یا دارونما (طعم‌دهنده‌ی بی اثر) را مصرف کردند، همچنین نوبت دوم مصرف پس از مرحله‌ی دوم آزمون و سومین نوبت پس از پایان کل چهار مرحله فعالیت مصرف شد. نمونه خون قبل از مصرف اولین نوبت مکمل و پس از اتمام کل چهار مرحله آزمون و نیز پس از یک ساعت بازگشت به حالت اولیه از آزمودنی‌ها گرفته شد. یافته‌ها: نتایج تحقیق با استفاده از روش تحلیل واریانس مکرر (3×2) نشان داد، مصرف مکمل ریبوز موجب کاهش معنی‌دار غلظت مالون‌دی آلدئید پس از انجام فعالیت شد (001/0=p)؛ اما با توجه به نتایج مشاهده شده، پاسخ ظرفیت ضداکسایشی تام (07/0=p) و گزانتین اکسیداز (71/0=p) به مصرف ریبوز مرتبط نبود. نتیجه‌گیری: به طور کلی به نظر می‌رسد که ریبوز احتمالاً با ورود در چرخه‌ی پنتوز فسفات و بازسازی ذخایر گلوتاتیون باعث جلوگیری از فشار اکسایشی شود.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The effect of acute ribose supplementation on Total Antioxidant Capacity (TAC), Lipid Peroxidation index (MDA) and Xanthine Oxidase (XO) after repeated intensive exercise
نویسندگان [English]
- ADEL DONYAEI 1
- Khosrow Ebrahim 2
- Hamid Rajabi 3
1 Assistant Professor of Department of Physical Education and Sport Sciences, Faculty of Physical Education, Shahrood University of Technology
2 Department of Sport Psychology, Faculty of Sport Sciences and Health, Shahid Beheshti University
3 Associate Professor of exercise physiology, Faculty of Physical Education and Sport Sciences, University of kharazmi Tehran
چکیده [English]
Introduction: Considering the various effects of ribose supplementation, the purpose of this study was to investigate the effect of ribose on total antioxidant capacity (TAC), lipid peroxidation index (MDA) and xanthine oxidase (XO) after repeated intensive exercise. Methodology: For this purpose 10 subjects with mean age (22±3 years) and BMI (23±2 kg/m2) voluntary participated in this study. Research was quasi-experimental and was done in double blind design. The protocol included 4 stages with 15 minutes of active rest; Each stage included three lower and upper extremity Wingate test and with alternative pattern with 1 min active rest between each test. First time ribose or placebo (0/1 gr per kg of body weight) were consumed. Also the second dose of supplement or placebo was give after the end of second stage of test and third dose was given after the completion of fourth stage. Blood sample was taken before and after and one hour after recovery. Findings: Data was analyzed with repeated measure (2×3) and the result showed that ribose supplementation decreased the concentration of MDA significantly after exercise (P=0/001). But regarding to observed result, TAC did not correlate to ribose consumption (P=0/07). Also XO was not related to ribose consumption (p=0/71). Conclusion: Generally it seems that ribose may prevent oxidative stress by entering the pentose phosphate cycle and restoring glutathione reserves
کلیدواژه‌ها [English]
- Ribose
- Oxidative Stress
- Repeated Intensive Exercise
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